Synthesis, characterization, and DNA-binding of chiral complexes delta- and lambda-[Ru(bpy)2(pyip)]2+

Chirality. 2009 Feb;21(2):276-83. doi: 10.1002/chir.20573.

Abstract

New chiral Ru(II) complexes delta and lambda-[Ru(bpy)(2)(pyip)](PF(6))(2) [(bpy = 2,2'-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, (1)H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Delta-enantiomer was stronger than that of Lambda-enantiomer. DNA-viscosity experiments provided evidence that both Delta- and Lambda-[Ru(bpy)(2)(pyip)](PF(6))(2) bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Circular Dichroism
  • DNA / metabolism*
  • DNA, Superhelical / metabolism
  • Deoxyribonucleases / metabolism
  • Electrons
  • Organometallic Compounds / chemical synthesis*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / metabolism*
  • Photolysis
  • Spectrometry, Fluorescence
  • Stereoisomerism
  • Viscosity

Substances

  • DNA, Superhelical
  • Organometallic Compounds
  • bis(2,2'-bipyridine)bis(2-(1-pyrenyl)-1H-imidazo(4,5-f)(1,10)phenanthroline)ruthenium(II)
  • DNA
  • calf thymus DNA
  • Deoxyribonucleases