Abstract
The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue of the GP64 envelope fusion protein of Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) F proteins were thought to be unable to functionally replace GP64 in the AcMNPV pseudotyping system. In the present study the F protein of Agrotis segetum GV (AgseGV) was identified experimentally as the first functional GP64 analogue from GVs. AgseF can rescue virion propagation and infectivity of gp64-null AcMNPV. The AgseF-pseudotyped AcMNPV also induced syncytium formation as a consequence of low-pH-induced membrane fusion.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Baculoviridae / metabolism
-
Cell Fusion
-
Cell Line
-
Chloramphenicol O-Acetyltransferase / genetics
-
Green Fluorescent Proteins / genetics
-
Hydrogen-Ion Concentration
-
Membrane Fusion
-
Models, Genetic
-
Moths / virology
-
Nucleopolyhedroviruses / genetics
-
Nucleopolyhedroviruses / metabolism*
-
Recombination, Genetic
-
Spodoptera / cytology
-
Transfection
-
Viral Envelope Proteins / genetics*
-
Viral Envelope Proteins / metabolism*
-
Viral Fusion Proteins / genetics
-
Viral Fusion Proteins / metabolism*
-
Virion / metabolism
-
Virus Replication
Substances
-
Viral Envelope Proteins
-
Viral Fusion Proteins
-
Green Fluorescent Proteins
-
Chloramphenicol O-Acetyltransferase