The investigation of putative agents, using an in vitro model, to prevent cavernosal smooth muscle dysfunction during low-flow priapism

BJU Int. 2008 Sep;102(8):988-92. doi: 10.1111/j.1464-410X.2008.07778.x. Epub 2008 Jun 28.

Abstract

Objective: To investigate the effect of putative agents for preventing irreversible smooth muscle dysfunction, using an in vitro model of low-flow priapism (a condition conventionally managed using a combination of corporal blood aspiration and instillation of alpha-adrenergic agonists), as failure of detumescence results in a high incidence of erectile dysfunction.

Materials and methods: We investigated the effects of several agents (N-acetylcysteine, BayK 8644, glutathione, digoxin, calcium and N(omega)-nitro-l-arginine methyl ester) on the recovery of smooth muscle tone after exposure to 4 h of a combination of hypoxia, glucopenia and acidosis in corpus cavernosum isolated from rabbit.

Results: After 4 h of ischaemia, none of the agents were able to prevent irreversible smooth muscle dysfunction.

Conclusion: Prolonged low-flow priapism leads to smooth muscle dysfunction and fibrosis within the corpus cavernosum. When alpha-adrenergic agents fail to reverse the condition, surgical intervention is required. We showed that the administration of novel agents, including antioxidants, does not prevent smooth muscle dysfunction.

MeSH terms

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
  • Acetylcysteine / pharmacology
  • Animals
  • Calcium / pharmacology
  • Digoxin / pharmacology
  • Glutathione / pharmacology
  • Male
  • Muscle Contraction / drug effects
  • Muscle Relaxation / drug effects
  • Muscle Tonus / drug effects
  • Muscle, Smooth / drug effects*
  • Muscle, Smooth / physiopathology
  • Muscular Diseases / etiology
  • Muscular Diseases / prevention & control*
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Penis / drug effects*
  • Penis / physiopathology
  • Priapism / complications*
  • Priapism / drug therapy
  • Priapism / physiopathology
  • Rabbits

Substances

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • Digoxin
  • Glutathione
  • Calcium
  • NG-Nitroarginine Methyl Ester
  • Acetylcysteine