Abstract
The inhibitory activities of the extracts of Cantharellus cibarius and isolated compounds were investigated in an enzyme-based ELISA NF-kappaB assay. Of the tested compounds, ergosterol, ergosterol peroxide and cerevisterol were noted to have the most potent inhibition of NF-kappaB activation. The ability of the active metabolites to inhibit the NF-kappaB translocation from the cytoplasm to the nucleus was assessed using a cell-based NF-kappaB assay. The isolated compounds were elucidated through the analysis of various spectroscopic methods.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Basidiomycota / chemistry*
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Ergosterol / analogs & derivatives
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Ergosterol / isolation & purification
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Ergosterol / pharmacology
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Fluorescent Antibody Technique, Indirect
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HeLa Cells / drug effects*
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HeLa Cells / metabolism
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HeLa Cells / pathology
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Humans
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / metabolism
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Phytosterols / isolation & purification
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Phytosterols / pharmacology
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
Substances
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NF-kappa B
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Phytosterols
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Plant Extracts
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cerevisterol
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ergosterol-5,8-peroxide
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Ergosterol