Abstract
We show an approach based on a combination of site-directed mutagenesis, NIL and multivalent host-guest interactions for the realization of engineered ordered functional arrays of purified components of the photosynthetic system, the membrane-bound LH2 complex. In addition to micrometer-scale patterned structures, we demonstrated the use of nanometer-scale hard NIL stamps to generate functional protein arrays approaching molecular dimensions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Glucosides / chemistry
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Light-Harvesting Protein Complexes / chemistry*
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Light-Harvesting Protein Complexes / genetics
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Microscopy, Atomic Force
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Mutagenesis, Site-Directed
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Nanotechnology / methods*
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Rhodobacter sphaeroides / chemistry
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Spectrometry, Fluorescence
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beta-Cyclodextrins / chemistry
Substances
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B800-850 light-harvesting complex, bacteria
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Bacterial Proteins
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Glucosides
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Light-Harvesting Protein Complexes
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beta-Cyclodextrins
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dodecyl maltoside
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betadex