Abstract
Experimental infection with the gamma-herpesvirus bovine herpesvirus 4 (BoHV-4) rarely establishes disease, yet BoHV-4 is commonly associated with uterine disease in cattle. Uterine disease involves co-infection with bacteria such as Escherichia coli, which stimulate the production of prostaglandin E(2) (PGE(2)) by endometrial cells. BoHV-4 replication depends on immediate early 2 (IE2) gene transactivation and, in the present study, PGE(2), E. coli or its lipopolysaccharide upregulated the IE2 gene promoter in uterine cells. Bacterial co-infection is important for BoHV-4 uterine disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cattle Diseases / genetics*
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Cells, Cultured
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Dinoprostone / metabolism
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Endometritis / microbiology
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Endometritis / virology
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Endometrium / microbiology
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Endometrium / virology
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Escherichia coli / physiology
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Escherichia coli Infections / complications
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Escherichia coli Infections / metabolism*
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Escherichia coli Infections / virology
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Female
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Genes, Immediate-Early*
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Herpesviridae Infections / genetics*
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Herpesvirus 4, Bovine / genetics*
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Lipopolysaccharides
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Promoter Regions, Genetic
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Stromal Cells / microbiology
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Stromal Cells / virology
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Transfection / methods
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Uterine Diseases / microbiology*
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Uterine Diseases / virology
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Uterus
Substances
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Lipopolysaccharides
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Dinoprostone