Association analysis of Krüppel-like factor 11 variants with type 2 diabetes in Pima Indians

J Clin Endocrinol Metab. 2008 Sep;93(9):3644-9. doi: 10.1210/jc.2008-0546. Epub 2008 Jul 1.

Abstract

Context: Krüppel-like factor 11 (KLF11) is a transcription factor of the zinc finger domain family that has been shown to regulate expression of the insulin gene. An initial study reported that a KLF11 variant predicting a Q62R was associated with type 2 diabetes (T2D) in French Caucasians; however, subsequent studies have failed to identify an association between this variant and T2D in subjects from a similar Northern-European ancestry.

Objective: We sought to determine whether the Q62R or other variants within KLF11 were associated with T2D in Pima Indians, a population with an extremely high prevalence of this disease.

Design, setting, and subjects: KLF11 was sequenced in 24 Pima Indians to identify potentially novel variants. There were 18 variants genotyped in a family-based sample of 1337 Pima Indians to analyze the linkage disequilibrium pattern of this gene and identify representative variants. Four representative variants were further genotyped in a population-based sample of 3501 full-heritage Pima Indians for association analyses. Among these subjects, 413 had undergone metabolic studies when they were nondiabetic to measure traits that predict T2D.

Results: Neither the Q62R nor any other common variant in KLF11 was associated with T2D in the Pima population. In addition, no variant was associated with insulin secretion or insulin-stimulated glucose disposal rate.

Conclusions: Common variation in KLF11 variation does not appear to influence the population-based risk for developing T2D among full-heritage Pima Indians. Thus, KLF11 is unlikely to play a major role in the etiology of T2D among this Native American population.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Adolescent
  • Adult
  • Apoptosis Regulatory Proteins
  • Cell Cycle Proteins / genetics*
  • Diabetes Mellitus, Type 2 / ethnology
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Gene Frequency
  • Genetic Linkage*
  • Genetic Predisposition to Disease
  • Haplotypes
  • Humans
  • Indians, North American / genetics*
  • Male
  • Middle Aged
  • Nuclear Family
  • Polymorphism, Single Nucleotide
  • Repressor Proteins / genetics*

Substances

  • Apoptosis Regulatory Proteins
  • Cell Cycle Proteins
  • KLF11 protein, human
  • Repressor Proteins