Abstract
We previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of DNA polymerase replicated DNA with increased fidelity. In this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased DNA replication fidelity.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Base Pair Mismatch
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DNA Replication*
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DNA-Directed DNA Polymerase / chemistry
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / isolation & purification
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DNA-Directed DNA Polymerase / metabolism*
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Exodeoxyribonucleases / chemistry
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Exodeoxyribonucleases / genetics
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Exodeoxyribonucleases / isolation & purification
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Exodeoxyribonucleases / metabolism*
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Herpesvirus 1, Human / enzymology*
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Herpesvirus 1, Human / genetics*
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Humans
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Kinetics
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Mutation*
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Nucleotides / metabolism
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Protein Structure, Tertiary
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Spodoptera / cytology
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Spodoptera / metabolism
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Viral Proteins / isolation & purification
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Viral Proteins / metabolism*
Substances
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Nucleotides
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Viral Proteins
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DNA-Directed DNA Polymerase
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Exodeoxyribonucleases
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DNA polymerase, Simplexvirus