Simultaneous multifocal, multiphoton, photon counting microscopy

Opt Express. 2008 Jul 7;16(14):10364-71. doi: 10.1364/oe.16.010364.

Abstract

We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Equipment Design
  • Image Processing, Computer-Assisted
  • Lasers*
  • Light
  • Microscopy / instrumentation*
  • Microscopy / methods
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Optics and Photonics*
  • Photons
  • Signal Processing, Computer-Assisted
  • Time Factors