Abstract
N-N-Staurosporine (STAR) inhibits in a dose dependent manner Tritiated-Thymidine (3H-TdR) incorporation in phytohemagglutinin (PHA) activated Peripheral Blood Mononuclear Cells (PBMC) treated with anti-HLA class I monoclonal antibody (MAb) 01.65 and its effect results competitive with MAb 01.65. Cytosolic and particulate Protein Kinase C (PKC) have been studied. Only when PKC particulate activity is no more detectable, the effect of STAR on 3H-TdR incorporation is evident.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / pharmacology*
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / pharmacology
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Cell Division
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Cells, Cultured
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Dose-Response Relationship, Drug
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Dose-Response Relationship, Immunologic
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HLA Antigens / immunology
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Histocompatibility Antigens Class I / immunology*
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Humans
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Lymphocyte Activation / drug effects*
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Lymphocyte Activation / immunology
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Phytohemagglutinins / pharmacology
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Protein Kinase C / antagonists & inhibitors*
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Protein Kinase C / metabolism
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Staurosporine
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T-Lymphocytes / drug effects*
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T-Lymphocytes / immunology
Substances
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Alkaloids
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Antibodies, Monoclonal
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HLA Antigens
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Histocompatibility Antigens Class I
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Phytohemagglutinins
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Protein Kinase C
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Staurosporine