Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis

J L Van Etten; R H Meints; D E Burbank; D Kuczmarski; D A Cuppels; L C Lane

doi:10.1016/0042-6822(81)90199-9

Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis

Virology. 1981 Sep;113(2):704-11. doi: 10.1016/0042-6822(81)90199-9.

Authors

J L Van Etten¹, R H Meints, D E Burbank, D Kuczmarski, D A Cuppels, L C Lane

Affiliation

¹ Department of Plant Pathology, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA.

PMID: 18635089
DOI: 10.1016/0042-6822(81)90199-9

Abstract

The isolation and characterization of a virus (designated HVCV) from the symbiotic Chlorella-like green alga present in Hydra viridis (Florida strain) are described. The large polyhedral viral particle sedimented at ca. 2600 S in sucrose density gradients and had a buoyant density in CsCl of 1.295 g/ml. The virus contained at least 19 polypeptides (MW range, 10,300 to 82,000) and the major polypeptide (MW, 46,000) was a glycoprotein. The viral genome consisted of a double-stranded DNA with a buoyant density in CsCl of 1.7114 g/ml (52% GC) and a molecular weight of ca. 136 x 10(6).