Abstract
Homology-driven proteomics is a major tool to characterize proteomes of organisms with unsequenced genomes. This paper addresses practical aspects of automated homology-driven protein identifications by LC-MS/MS on a hybrid LTQ Orbitrap mass spectrometer. All essential software elements supporting the presented pipeline are either hosted at the publicly accessible web server, or are available for free download.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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Chromatography, Liquid / methods
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Computational Biology / methods
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Databases, Protein
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Genome
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Insect Proteins / chemistry
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Internet
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Mass Spectrometry / methods*
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Molecular Sequence Data
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Peptide Mapping
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Plant Proteins / chemistry
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Proteins / analysis*
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Proteins / chemistry
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Proteomics / methods*
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Software
Substances
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Insect Proteins
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Plant Proteins
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Proteins