Abstract
The CHCl(3)-soluble fraction of 70% EtOH extract of the flower of Kayea assamica completely killed human pancreatic PANC-1 cancer cells preferentially under nutrient-deprived conditions at 1 microg/mL. Bioassay-guided fractionation and isolation afforded two novel compounds, kayeassamins A (1) and B (2). Their structures were elucidated using extensive spectroscopic methods and the modified Mosher method. Each compound showed 100% preferential cytotoxicity (PC(100)) against PANC-1 cells under nutrient-deprived conditions at 1 microM. Furthermore, both compounds inhibited the migration of PANC-1 cells in the wound closure assay.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antineoplastic Agents, Phytogenic / isolation & purification*
-
Antineoplastic Agents, Phytogenic / pharmacology
-
Cell Line, Tumor
-
Clusiaceae / metabolism*
-
Coumarins / isolation & purification*
-
Coumarins / pharmacology
-
Drug Design
-
Drug Screening Assays, Antitumor*
-
Flowers / metabolism
-
Humans
-
Magnetic Resonance Spectroscopy
-
Models, Chemical
-
Monoterpenes / isolation & purification*
-
Monoterpenes / pharmacology
-
Myanmar
-
Pancreatic Neoplasms / drug therapy*
-
Plant Extracts / pharmacology*
Substances
-
Antineoplastic Agents, Phytogenic
-
Coumarins
-
Monoterpenes
-
Plant Extracts
-
kayeassamin A
-
kayeassamin B