Abstract
Growth of Ralstonia solanacearum strain OE1-1 in roots after invasion is required for virulence. An Arg740Cys substitution in sigma(70) of OE1-1 resulted in loss of in planta growth and virulence. The negative dominance of mutant sigma(70) over the wild-type protein suggested that the amino acid substitution may affect the in planta growth of OE1-1, leading to a lack of virulence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution*
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DNA Transposable Elements
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DNA-Directed RNA Polymerases / genetics*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Escherichia coli / pathogenicity
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Genes, Bacterial
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Molecular Sequence Data
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Mutagenesis, Insertional
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Nicotiana / microbiology
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Plant Diseases / microbiology*
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Plant Roots / microbiology
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Plasmids
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RNA, Bacterial / genetics
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Ralstonia solanacearum / enzymology
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Ralstonia solanacearum / genetics*
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Ralstonia solanacearum / growth & development
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Ralstonia solanacearum / pathogenicity*
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Sigma Factor / genetics*
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Virulence / genetics
Substances
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DNA Transposable Elements
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RNA, Bacterial
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Sigma Factor
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RNA polymerase sigma 70
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DNA-Directed RNA Polymerases
Associated data
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GENBANK/AB358977
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GENBANK/AB445124