Abstract
Affinity-matured human antibodies have demonstrated efficacy as countermeasures for exposure to botulinum neurotoxin (BoNT), which is the cause of the disease botulism category A select bioterror agent. Little is known, however, about the potential role of natural (un-mutated) antibodies in the protective immune response to BoNT. Here we describe the cloning of two human IgM antibodies that bind serotype A BoNT. Both are un-mutated IgM antibodies, consistent with an origin in naive B cells. One of the antibodies is able to fully neutralize a lethal dose of serotype A BoNT in vivo. These results suggest that the natural human antibody repertoire may play a role in protection from exposure to biological toxins.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Antibodies, Bacterial / administration & dosage
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Antibodies, Bacterial / metabolism
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Antibodies, Bacterial / physiology*
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Binding Sites, Antibody
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Botulinum Toxins / antagonists & inhibitors
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Botulinum Toxins / immunology*
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Botulinum Toxins / metabolism
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Botulinum Toxins, Type A / antagonists & inhibitors
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Botulinum Toxins, Type A / immunology*
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Botulinum Toxins, Type A / metabolism
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Botulism / immunology
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Botulism / prevention & control
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Cell Line, Tumor
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Cells, Cultured
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Clostridium botulinum / immunology*
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Humans
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Hybridomas
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Immunoglobulin M / administration & dosage
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Immunoglobulin M / metabolism
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Immunoglobulin M / physiology*
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Molecular Sequence Data
Substances
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Antibodies, Bacterial
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Immunoglobulin M
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rimabotulinumtoxinB
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Botulinum Toxins
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Botulinum Toxins, Type A