Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu

Gene. 1991 Jun 15;102(1):123-7. doi: 10.1016/0378-1119(91)90550-u.

Abstract

We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal antibody. The nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-Da protein. Computer-aided sequence comparison indicated that the gene codes for elongation factor Tu.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, Bacterial / genetics
  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Genetic Variation
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Mycoplasma / genetics*
  • Peptide Elongation Factor Tu / biosynthesis
  • Peptide Elongation Factor Tu / genetics*
  • Plasmids / genetics
  • Recombinant Proteins / biosynthesis
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Recombinant Proteins
  • Peptide Elongation Factor Tu

Associated data

  • GENBANK/M57675
  • GENBANK/S39290
  • GENBANK/S39297
  • GENBANK/S47470
  • GENBANK/S47553
  • GENBANK/S70115
  • GENBANK/S70117
  • GENBANK/S70121
  • GENBANK/S70125
  • GENBANK/S70128
  • GENBANK/S70130