Comparison of laboratory-based and phylogenetic methods to distinguish between Haemophilus influenzae and H. haemolyticus

J Microbiol Methods. 2008 Oct;75(2):369-71. doi: 10.1016/j.mimet.2008.06.023. Epub 2008 Jul 3.

Abstract

New methods to distinguish between nontypeable Haemophilus influenzae and nonhemolytic H. haemolyticus were compared. The results of iga variable region hybridization to dotblots and library-on-a-slide microarrays were more similar to a "gold standard" multigenephylogenetic tree than iga-conserved region hybridization or P6 7F3 epitope immunoblots.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry
  • Carrier State / microbiology*
  • Child, Preschool
  • Epitopes / analysis
  • Haemophilus / classification*
  • Haemophilus / genetics
  • Haemophilus / isolation & purification
  • Haemophilus Vaccines / chemistry
  • Haemophilus influenzae / classification*
  • Haemophilus influenzae / genetics
  • Haemophilus influenzae / isolation & purification
  • Humans
  • Immunoblotting / methods*
  • Nucleic Acid Hybridization / methods
  • Oligonucleotide Array Sequence Analysis / methods*
  • Pharynx / microbiology
  • Phylogeny*
  • Serine Endopeptidases / genetics*

Substances

  • Bacterial Outer Membrane Proteins
  • Epitopes
  • Haemophilus Vaccines
  • P6 outer membrane protein, Haemophilus
  • Serine Endopeptidases
  • IgA-specific serine endopeptidase