Abstract
Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Acetyltransferases / chemistry
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Acetyltransferases / genetics
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Acetyltransferases / metabolism*
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Alleles
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Chondroitin Sulfate Proteoglycans / chemistry
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Chondroitin Sulfate Proteoglycans / genetics
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Chondroitin Sulfate Proteoglycans / metabolism*
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Chromatids / physiology*
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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Chromosomes, Fungal / physiology*
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DNA Repair
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DNA Replication
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DNA, Fungal / metabolism
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Mutation
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism
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S Phase
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Suppression, Genetic
Substances
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Cell Cycle Proteins
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Chondroitin Sulfate Proteoglycans
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Chromosomal Proteins, Non-Histone
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DNA, Fungal
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Nuclear Proteins
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Protein Subunits
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RAD61 protein, S cerevisiae
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SMC3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Acetyltransferases
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ECO1 protein, S cerevisiae