A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells

Huiping Lou; Yanfeng Gao; Mingxia Zhai; Yuanming Qi; Lixiang Chen; Hong Lv; Jibing Yu; Yongxin Li

doi:10.1016/j.bmcl.2008.07.017

A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells

Bioorg Med Chem Lett. 2008 Aug 15;18(16):4633-7. doi: 10.1016/j.bmcl.2008.07.017. Epub 2008 Jul 10.

Authors

Huiping Lou¹, Yanfeng Gao, Mingxia Zhai, Yuanming Qi, Lixiang Chen, Hong Lv, Jibing Yu, Yongxin Li

Affiliation

¹ Department of Bioengineering, Zhengzhou University, Science Road 100, Zhengzhou 450001, China.

PMID: 18656352
DOI: 10.1016/j.bmcl.2008.07.017

Abstract

A series of novel peptides from various motifs of Asterina pectinifera cyclin B and their derivatives conjugated to HIV-Tat(49-57) were designed and synthesized. Their bioactivities on two human cancer cell lines were determined. Among them, Tat-a5 (KAQIRAMECNILGRKKRRQRRR) exhibited significant cytotoxic effects on cancer cell lines EC-9706 and HCT-116. Tat-a5 could arrest cancer cells at G(2)/M phase and make them apoptotic. Our results suggested that Tat-a5 could be a novel leading peptide with anticancer activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antineoplastic Agents / pharmacology*
Apoptosis*
Asterina / metabolism*
Cell Cycle / drug effects
Cell Line, Tumor
Chemistry, Pharmaceutical / methods*
Cyclin B / chemistry*
Dose-Response Relationship, Drug
Drug Design
Humans
Molecular Sequence Data
Peptide Fragments / chemistry*
Peptides / chemistry
tat Gene Products, Human Immunodeficiency Virus / chemistry*

Substances

Antineoplastic Agents
Cyclin B
Peptide Fragments
Peptides
tat Gene Products, Human Immunodeficiency Virus
tat peptide (49-57), Human immunodeficiency virus 1