Papaya ringspot virus-P: characteristics, pathogenicity, sequence variability and control

Mol Plant Pathol. 2008 May;9(3):269-80. doi: 10.1111/j.1364-3703.2008.00467.x.

Abstract

Taxonomy: Papaya ringspot virus (PRSV) is an aphid-transmitted plant virus belonging to the genus Potyvirus, family Potyviridae, with a positive sense RNA genome. PRSV isolates belong to either one of two major strains, P or W. The P strains infect both papaya and cucurbits whereas the W strains infect only cucurbits.

Geographical distribution: PRSV-P is found in all major papaya-growing areas.

Physical properties: Virions are filamentous, non-enveloped and flexuous measuring 760-800 x 12 nm. Virus particles contain 94.5% protein and 5.5% nucleic acid. The protein component consists of the virus coat protein (CP), which has a molecular weight of about 36 kDa as estimated by Western blot analysis. Density of the sedimenting component in purified PRSV preparations is 1.32 g/cm(3) in CsCl.

Genome: The PRSV genome consists of a unipartite linear single-stranded positive sense RNA of 10 326 nucleotides with a 5' terminus, genome-linked protein, VPg.

Transmission: The virus is naturally transmitted via aphids in a non-persistent manner. Both the CP and helper component (HC-Pro) are required for vector transmission. This virus can also be transmitted mechanically, and is typically not seed-transmitted.

Hosts: PRSV has a limited number of hosts belonging to the families Caricaceae, Chenopodiaceae and Cucurbitaceae. Propagation hosts are: Carica papaya, Cucurbita pepo and Cucumis metuliferus cv. accession 2459. Local lesion assay hosts are: Chenopodium quinoa and Chenopodium amaranticolor.

Control: Two transgenic papaya varieties, Rainbow and SunUp, with engineered resistance to PRSV have been commercially grown in Hawaii since 1998. Besides transgenic resistance, tolerant varieties, cross-protection and other cultural practices such as isolation and rogueing of infected plants are used to manage the disease.

Virus code: 00.057.0.01.045.

Virus accession number: 57010045.

Useful link: http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/57010045.htm.

MeSH terms

  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism
  • Carica / virology*
  • Genome, Viral / genetics*
  • Phylogeny
  • Plant Diseases / virology*
  • Plant Viruses / classification
  • Plant Viruses / genetics*
  • Plant Viruses / pathogenicity
  • RNA, Viral / genetics
  • Virulence / genetics

Substances

  • Capsid Proteins
  • RNA, Viral
  • coat protein, Papaya ringspot virus