Bovine serum albumin contained in culture medium used in artificial insemination is an important anaphylaxis risk factor

Fertil Steril. 2008 Nov;90(5):2013.e17-9. doi: 10.1016/j.fertnstert.2008.05.055. Epub 2008 Aug 16.

Abstract

Objective: To analyze the cause of the anaphylactic reaction after a standard artificial insemination process in a patient diagnosed with asthma.

Design: Case report.

Setting: Residencia Sanitaria Virgen de la Arrixaca (Murcia, Spain) and University of the Basque Country (Vitoria, Spain).

Patient(s): A 30-year-old woman with a previous medical history compatible with respiratory allergy who suffered an anaphylactic reaction after an artificial insemination with spermatozoids in capable medium (Upgraded B2 INRA medium; Laboratories CCD, Paris, France).

Intervention(s): Cutaneous tests and specific IgE levels to inhalant allergens, grass and Olea pollens, and insemination medium were performed.

Main outcome measure(s): Specific IgE levels to mammal epithelia and bovine serum albumin (BSA).

Result(s): Skin prick tests were positive for inhalant allergens such as mites, cat, dog, horse, and rabbit epithelia, grasses and Olea pollens, and the insemination medium. The beta-lactamic tests were negative. The determination of specific IgE demonstrated positive values to mammal epithelia and mammal serum albumins including BSA.

Conclusion(s): We report a case of an anaphylactic reaction to the BSA included in the insemination culture medium induced by a subclinical sensitivity to serum albumins of mammal epithelia. A previous testing with the medium is recommended and specific testing might be needed in women who have a history of animal epithelium allergies.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Allergens*
  • Anaphylaxis / chemically induced*
  • Anaphylaxis / immunology
  • Asthma / complications*
  • Culture Media / adverse effects*
  • Female
  • Humans
  • Immunoglobulin E / blood
  • Infertility / complications
  • Infertility / therapy*
  • Insemination, Artificial / adverse effects*
  • Serum Albumin, Bovine / adverse effects*
  • Serum Albumin, Bovine / immunology
  • Skin Tests

Substances

  • Allergens
  • Culture Media
  • Serum Albumin, Bovine
  • Immunoglobulin E