Abstract
We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Androgens / analysis*
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Biological Assay / instrumentation*
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Biosensing Techniques / instrumentation*
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Equipment Design
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Equipment Failure Analysis
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Immunoassay / instrumentation*
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Microscopy, Fluorescence, Multiphoton / instrumentation*
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Nonlinear Dynamics
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Photometry / instrumentation*
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Refractometry / instrumentation*
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Transducers