Immunoexpression of vascular endothelial growth factor in periapical granulomas, radicular cysts, and residual radicular cysts

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Dec;106(6):896-902. doi: 10.1016/j.tripleo.2008.06.028. Epub 2008 Aug 28.

Abstract

Objective: Our aim was to assess and compare the immunoexpression of vascular endothelial growth factor (VEGF) in periapical granulomas (PGs), radicular cysts (RCs), and residual radicular cysts (RRCs), relating it to the angiogenic index and the intensity of the inflammatory infiltrate.

Study design: Twenty PGs, 20 RCs, and 10 RRCs were evaluated by immunohistochemistry using anti-VEGF antibody. Angiogenic index was determined by microvessel count (MVC) using anti-von Willebrand factor antibody.

Results: The PGs and RCs showed higher expression of VEGF than the RRCs. Lesions presenting few inflammatory infiltrate revealed the lowest immunoexpression of VEGF (P < .05). Irrespective of the intensity of the inflammatory infiltrate, most of the RCs and RRCs showed moderate to strong epithelial expression of VEGF. Lesions showing dense inflammatory infiltrate presented higher MVC indices (P < .05). VEGF expression and MVC did not reveal a significant correlation (P > .05).

Conclusions: VEGF is present in periapical inflammatory lesions but at a lower level in RRCs. The expression of this proangiogenic factor is closely related to the intensity of the inflammatory infiltrate in these lesions.

MeSH terms

  • Capillary Permeability
  • Cyst Fluid
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Microvessels
  • Neovascularization, Pathologic
  • Neutrophil Infiltration
  • Periapical Granuloma / metabolism*
  • Radicular Cyst / blood supply
  • Radicular Cyst / metabolism*
  • Statistics, Nonparametric
  • Vascular Endothelial Growth Factor A / biosynthesis*

Substances

  • Vascular Endothelial Growth Factor A