Abstract
Two new phenyl glycosides, mangliesides A and B (1, 2), a new ionol glycoside, manglieside C (3), two new lignan glycosides, mangliesides D and E (4, 5), were isolated from the leaves of Manglietia phuthoensis, along with two known lignans, 3-methoxymagnolol (6) and obovatol (7). Their structures were established by means of 1D and 2D NMR, electrospray ionization (ESI)-MS and HR-ESI-MS experiments. Among them, compounds 2 and 5 significantly (p<0.05) increased the growth and differentiation of osteoblastic MC3T3-E1 cells in vitro.
MeSH terms
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3T3 Cells
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Alkaline Phosphatase / metabolism
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Animals
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Bone Density Conservation Agents / isolation & purification*
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Bone Density Conservation Agents / pharmacology*
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Calcium / metabolism
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Cell Differentiation / drug effects
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Cell Survival / drug effects
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Collagen / analysis
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Collagen / biosynthesis
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Estradiol / chemistry
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Estradiol / pharmacology
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Hydrolysis
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Magnetic Resonance Spectroscopy
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Magnolia / chemistry*
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Mice
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Models, Molecular
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Molecular Conformation
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Osteoblasts / drug effects*
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Plant Leaves / chemistry
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Spectrometry, Mass, Electrospray Ionization
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Spectrophotometry, Infrared
Substances
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Bone Density Conservation Agents
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Estradiol
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Collagen
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Alkaline Phosphatase
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Calcium