Abstract
We report here on the use of a Sindbis virus-based DNA-launch RNA replicon vector (pSIN-HA) that expresses influenza hemagglutinin (HA) as an immunogen. Immunization of mice with pSIN-HA generated anti-HA antibody and CTL responses and resulted in lower lung viral titers after influenza challenge when compared to controls. Importantly, immunization with a low dose of pSIN-HA mediated significantly reduced lung viral titers following challenge at 43 weeks after the final immunization. In contrast, immunization with a non-replicon DNA vector expressing HA failed to mediate reduced lung viral titer at the same dose. This demonstrated the dose-sparing capacity of the SIN vector system and its ability to stimulate long-term memory responses, properties that are highly desirable in any vaccine formulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Cricetinae
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Female
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Genetic Vectors*
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism
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Immunization
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / immunology*
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Influenza Vaccines / administration & dosage*
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Lung / virology
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Mice
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Mice, Inbred BALB C
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Orthomyxoviridae Infections / immunology*
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Orthomyxoviridae Infections / prevention & control*
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Orthomyxoviridae Infections / virology
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Plasmids
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Replicon
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Sindbis Virus / genetics*
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Sindbis Virus / immunology
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T-Lymphocytes, Cytotoxic / immunology
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Vaccination
Substances
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Antibodies, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines