Abstract
Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides, siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to microg range) to living neurons in a reasonable period of time.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Antibodies / administration & dosage*
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Cell Survival / drug effects
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Cell Survival / physiology
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Cells, Cultured
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DNA-Binding Proteins / metabolism
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Green Fluorescent Proteins / biosynthesis
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Hippocampus / cytology*
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Mice
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Microinjections / methods*
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Neurons / drug effects*
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Neurons / physiology
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Nuclear Proteins / metabolism
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Nucleic Acids / administration & dosage*
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Oligodeoxyribonucleotides, Antisense / administration & dosage
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Peptides / administration & dosage*
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Receptors, GABA-A / genetics
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Receptors, GABA-A / metabolism
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Ubiquitin-Protein Ligases
Substances
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Antibodies
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DNA-Binding Proteins
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Nuclear Proteins
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Nucleic Acids
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Oligodeoxyribonucleotides, Antisense
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Peptides
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Receptors, GABA-A
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Green Fluorescent Proteins
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Trim27 protein, mouse
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Ubiquitin-Protein Ligases