Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2)

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):847-50. doi: 10.1107/S174430910802575X. Epub 2008 Aug 20.

Abstract

A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 A resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 29.67, b = 67.00, c = 34.43 A, alpha = gamma = 90.00, beta = 94.26 degrees .

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism
  • Chromatography, Agarose
  • Cloning, Molecular*
  • Crystallization
  • Crystallography, X-Ray
  • Metals / chemistry
  • Metals / metabolism
  • Molecular Sequence Data
  • Oxidative Stress / genetics
  • Phosphates / metabolism
  • Streptomyces coelicolor / chemistry*
  • Streptomyces coelicolor / genetics
  • Streptomyces coelicolor / metabolism

Substances

  • Bacterial Proteins
  • Metals
  • Phosphates