Abstract
It is currently unclear if there are modified DNA bases in Trypanosoma brucei other than J-base. We identify herein a cytosine-5 DNA methyltransferase gene and report the presence and location of 5-methylcytosine in genomic DNA. Our data demonstrate that African trypanosomes contain a functional cytosine DNA methylation pathway.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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5-Methylcytosine / metabolism*
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Amino Acid Sequence
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Animals
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DNA (Cytosine-5-)-Methyltransferases / genetics
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DNA (Cytosine-5-)-Methyltransferases / metabolism
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DNA Methylation*
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DNA, Protozoan / genetics
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DNA, Protozoan / metabolism*
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Molecular Sequence Data
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Protozoan Proteins / genetics
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Protozoan Proteins / metabolism
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Sequence Alignment
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Trypanosoma brucei brucei / enzymology
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Trypanosoma brucei brucei / genetics
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Trypanosoma brucei brucei / metabolism*
Substances
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DNA, Protozoan
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Protozoan Proteins
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5-Methylcytosine
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DNA (Cytosine-5-)-Methyltransferases