Abstract
By loading doxorubicin (DOX) on 5-carboxyl-fluorescein (FAM) labeled AGKGTPSLETTP peptide (A54) coupled starch-coated iron oxide nanoparticles (SIONs), we prepared a novel aqueous drug delivery system with both magnetic and biomolecular targeting, which was specific to human hepatocellular carcinoma cell line BEL-7402. The saturated extent of adsorption reached 2.0 mg DOX/mg A54-SIONs at 28 degrees C, which provided a rather high dose of DOX loading for application. Tests in vitro demonstrated the specificity of DOX-loaded A54-SIONs to BEL-7402 cells. The microscopy images proved that DOX-loaded A54-SIONs were successfully targeted to tumor tissue of nude mice with an external magnetic field in vivo. MTT assay showed higher cytostatic effect of DOX-loaded A54-SIONs to hepatocellular carcinoma cells BEL-7402 than that of DOX-loaded SIONs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibiotics, Antineoplastic / administration & dosage
-
Biocompatible Materials / chemistry
-
Biocompatible Materials / isolation & purification
-
Cell Line, Tumor
-
Doxorubicin / administration & dosage
-
Drug Carriers / chemistry
-
Drug Carriers / isolation & purification*
-
Drug Delivery Systems*
-
Fluoresceins
-
Fluorescent Dyes
-
Humans
-
Liver Neoplasms, Experimental / drug therapy
-
Liver Neoplasms, Experimental / pathology
-
Magnetics
-
Materials Testing
-
Metal Nanoparticles / chemistry
-
Mice
-
Mice, Nude
-
Microscopy, Fluorescence
-
Neoplasm Transplantation
-
Oligopeptides / chemistry
-
Spectroscopy, Fourier Transform Infrared
-
Transplantation, Heterologous
Substances
-
Antibiotics, Antineoplastic
-
Biocompatible Materials
-
Drug Carriers
-
Fluoresceins
-
Fluorescent Dyes
-
Oligopeptides
-
4-carboxyfluorescein
-
Doxorubicin