Abstract
Effects of antisense Ki-67 cDNA transfection on breast cancer cells were investigated in this study. Transfection of antisense Ki-67 cDNA resulted in a 70%-80% reduction in proliferation of MDA-MB-435s, cells which highly expressed Ki-67 mRNA and protein. Transwell assay showed that mobility and invasion capability was dramatically inhibited by 50%-60%, and cell cycle analysis displayed a higher proportion in G(2)/M and G(0)/G(1) phases accompanied by remarkably increased ratio of apoptotic cells. Our results suggested that antisense Ki-67 cDNA vector treatment might be an important potential option in the anticancer therapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis
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Breast Neoplasms / pathology*
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Carcinoma, Ductal, Breast / pathology*
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Cell Cycle
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Cell Division
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Cell Line, Tumor / pathology
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Cell Movement
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DNA, Antisense / genetics
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DNA, Complementary / genetics
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Estrogens
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Humans
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Ki-67 Antigen / genetics
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Ki-67 Antigen / physiology*
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Neoplasm Invasiveness
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Neoplasm Proteins / antagonists & inhibitors
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Neoplasm Proteins / genetics
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Neoplasm Proteins / physiology*
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Neoplasms, Hormone-Dependent / pathology
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / genetics
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Recombinant Fusion Proteins / physiology
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Transfection
Substances
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DNA, Antisense
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DNA, Complementary
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Estrogens
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Ki-67 Antigen
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Fusion Proteins