Abstract
Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / genetics
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Antigens, CD / physiology*
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Chlorocebus aethiops
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Green Fluorescent Proteins / genetics
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Measles Vaccine / immunology*
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Measles virus / genetics*
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Mice
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RNA, Viral / biosynthesis
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RNA-Dependent RNA Polymerase / genetics*
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RNA-Dependent RNA Polymerase / immunology
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology*
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Signaling Lymphocytic Activation Molecule Family Member 1
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Vaccines, Attenuated / immunology
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Vaccines, Synthetic / immunology*
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Vero Cells
Substances
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Antigens, CD
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Measles Vaccine
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RNA, Viral
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Receptors, Cell Surface
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Vaccines, Attenuated
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Vaccines, Synthetic
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Signaling Lymphocytic Activation Molecule Family Member 1
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RNA-Dependent RNA Polymerase