Abstract
GDP-2-, 3-, 4- or 6-azidomannoses can be successfully prepared from the corresponding azidomannose-1-phosphates and GTP using the enzyme GDP-Mannosepyrophosphorylase (GDP-ManPP) from Salmonella enterica and may serve as useful probes for mannosyltransferase activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Conformation
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Enzyme Activation
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Guanosine Diphosphate Mannose / analogs & derivatives*
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Guanosine Diphosphate Mannose / chemical synthesis*
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Guanosine Diphosphate Mannose / chemistry
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Guanosine Triphosphate / chemistry
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Mannosyltransferases / analysis
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Mannosyltransferases / chemistry*
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Molecular Probes / chemistry*
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Recombinant Proteins / chemistry
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Salmonella enterica / enzymology
Substances
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Molecular Probes
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Recombinant Proteins
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Guanosine Diphosphate Mannose
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Guanosine Triphosphate
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Mannosyltransferases