The human protease inhibitor cystatin C is an activating cofactor for the streptococcal cysteine protease IdeS

Chem Biol. 2008 Sep 22;15(9):960-8. doi: 10.1016/j.chembiol.2008.07.021.

Abstract

Human cystatin C is considered the physiologically most important inhibitor of endogenous papain-like cysteine proteases. We present here an unexpected function of cystatin C. Instead of acting as an inhibitor, cystatin C acts as a facultative, endogenous cofactor for the papain-like IgG-cleaving enzyme IdeS of the human pathogen Streptococcus pyogenes. IdeS activity is not dependent on cystatin C, but is significantly enhanced in the presence of cystatin C. We report a protease inhibitor that accelerates the activity of its putative target protease and a unique example of how a host protease inhibitor is "hijacked" by a bacterial protease to increase its activity. This finding has important implications for the view on protease-inhibitor interactions, and is relevant to consider in the therapeutic use of protease inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Cystatin C
  • Cystatins / metabolism*
  • Cysteine Endopeptidases / metabolism*
  • Drug Evaluation, Preclinical
  • Enzyme Activation
  • Humans
  • Hydrolysis
  • Immunoglobulin G / immunology
  • Immunoglobulin G / metabolism
  • Kinetics
  • Protease Inhibitors / metabolism*
  • Protein Binding
  • Streptococcus / enzymology*
  • Surface Plasmon Resonance

Substances

  • Bacterial Proteins
  • CST3 protein, human
  • Cystatin C
  • Cystatins
  • Immunoglobulin G
  • Mac-1-like protein, Streptococcus
  • Protease Inhibitors
  • Cysteine Endopeptidases