Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2 standard method

Luis Ostrosky-Zeichner; John H Rex; Michael A Pfaller; Daniel J Diekema; Barbara D Alexander; David Andes; Steven D Brown; Vishnu Chaturvedi; Mahmoud A Ghannoum; Cindy C Knapp; Daniel J Sheehan; Thomas J Walsh

doi:10.1128/AAC.00420-08

Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2 standard method

Antimicrob Agents Chemother. 2008 Nov;52(11):4175-7. doi: 10.1128/AAC.00420-08. Epub 2008 Sep 22.

Authors

Luis Ostrosky-Zeichner¹, John H Rex, Michael A Pfaller, Daniel J Diekema, Barbara D Alexander, David Andes, Steven D Brown, Vishnu Chaturvedi, Mahmoud A Ghannoum, Cindy C Knapp, Daniel J Sheehan, Thomas J Walsh

Affiliation

¹ University of Texas Medical School at Houston, Texas 77030, USA. [email protected]

Abstract

We investigated if CLSI M27-A2 Candida species breakpoints for fluconazole MIC are valid when read at 24 h. Analysis of a data set showed good correlation between 48- and 24-h MICs, as well as similar outcomes and pharmacodynamic efficacy parameters, except for isolates in the susceptible dose-dependent category, such as Candida glabrata.

Publication types

Comparative Study

MeSH terms

Animals
Antifungal Agents / administration & dosage
Antifungal Agents / pharmacology*
Candida / drug effects*
Candida / isolation & purification
Candidiasis / drug therapy
Candidiasis / microbiology
Databases, Factual
Fluconazole / administration & dosage
Fluconazole / pharmacology*
Humans
Microbial Sensitivity Tests / methods
Microbial Sensitivity Tests / standards*
Microbial Sensitivity Tests / statistics & numerical data
Models, Biological
Time Factors

Substances

Antifungal Agents
Fluconazole