[Detection of hybridization probes using antibodies to DNA adducts with cis- and trans-diaminodichloroplatinum (II). I. Immunochemical characteristics of DNA modified by cis- and trans-diaminodichloroplatinum (II)]

V I Kiseleva; O Vrana; I Brabets; A M Poverennyĭ

[Detection of hybridization probes using antibodies to DNA adducts with cis- and trans-diaminodichloroplatinum (II). I. Immunochemical characteristics of DNA modified by cis- and trans-diaminodichloroplatinum (II)]

Mol Biol (Mosk). 1991 Mar-Apr;25(2):503-7.

[Article in Russian]

Authors

V I Kiseleva, O Vrana, I Brabets, A M Poverennyĭ

PMID: 1881396

Abstract

Rabbit antisera elicited against calf thymus DNA modified cis- and trans-diaminedichlorplatinum II (DDP) [Pt/nucleotide ratio - 0.1] contain antibodies specific for Pt-modified DNA immunogen. A specific antibody against DNA-cisDDP and DNA-transDDP was obtained by affinity chromatography. In the ELISA, 3.10(-1 6) mol Pt per micrograms DNA was determined. The epitope structure was determined by using DNA modified with cisDDP and transDDP.

Publication types

English Abstract

MeSH terms

Animals
Cattle
Cisplatin / pharmacology*
DNA Damage*
Immunohistochemistry
Nucleic Acid Hybridization
Stereoisomerism
Thymus Gland / metabolism

Substances

transplatin
Cisplatin