Intratumoral expression of macrophage migration inhibitory factor is correlated with serum C-reactive protein and interleukin-6 in patients with non-small cell lung cancer

Surg Today. 2008;38(10):921-5. doi: 10.1007/s00595-008-3765-z. Epub 2008 Sep 27.

Abstract

Purpose: To investigate if macrophage migration inhibitory factor (MIF) affects systemic inflammatory response in patients with non-small cell lung cancer (NSCLC), we studied the relationship between intratumoral MIF expression and serum levels of C-reactive protein (CRP) and interleukin-6 (IL-6).

Methods: mRNAs for MIF and beta-actin, and 18S ribosomal RNA derived from tumor-bearing and nontumor-bearing tissues were quantified by a quantitative reverse transcription-polymerase chain reaction in 35 patients with NSCLC. Spearman's test was used to examine the correlation between the quantities of RNAs and the preoperative serum levels of CRP and IL-6 in the corresponding patients.

Results: The intratumoral level of MIF mRNA was significantly correlated with the serum levels of CRP (rho = 0.542, P = 0.0016) and IL-6 (rho = 0.532, P = 0.0173). The level of MIF mRNA in the non-tumor-bearing lung also showed a positive, but relatively weak correlation with the serum CRP level (rho = 0.418, P = 0.0148).

Conclusion: Macrophage migration inhibitory factor expression in the tumor site may affect the systemic inflammatory response in patients with NSCLC.

MeSH terms

  • C-Reactive Protein / metabolism*
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Female
  • Humans
  • Interleukin-6 / metabolism*
  • Lung Neoplasms / metabolism*
  • Macrophage Migration-Inhibitory Factors / metabolism*
  • Male
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Interleukin-6
  • Macrophage Migration-Inhibitory Factors
  • RNA, Messenger
  • C-Reactive Protein