Abstract
A full-length cDNA clone for sepiapterin reductase, an enzyme involved in tetrahydrobiopterin biosynthesis, was isolated from a human liver cDNA library by plaque hybridization. The nucleotide sequence of hSPR 8-25, which contained an entire coding region of the enzyme, was determined. The clone encoded a protein of 261 amino acids with a calculated molecular mass of 28,047 daltons. The predicted amino acid sequence of human sepiapterin reductase showed a 74% identity with the rat enzyme. We further found a striking homology between human SPR and carbonyl reductase, estradiol 17 beta-dehydrogenase, and 3 beta-hydroxy-5-ene steroid dehydrogenase, especially in their N-terminal region.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Biopterins / analogs & derivatives*
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Biopterins / biosynthesis
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Cloning, Molecular
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DNA / genetics*
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DNA / isolation & purification
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Escherichia coli / genetics
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Gene Library
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Humans
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Liver / enzymology
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotide Probes
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Polymerase Chain Reaction / methods
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Rats
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Sequence Homology, Nucleic Acid
Substances
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Oligonucleotide Probes
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Biopterins
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DNA
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Alcohol Oxidoreductases
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sepiapterin reductase
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sapropterin
Associated data
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GENBANK/M74785
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GENBANK/M74786
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GENBANK/M74787
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GENBANK/M74788
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GENBANK/M74790
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GENBANK/M76231
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GENBANK/M81108
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GENBANK/M81109
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GENBANK/S57525
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GENBANK/S57567