Abstract
To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.
MeSH terms
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Animals
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Brucella Vaccine / genetics*
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Brucella Vaccine / immunology*
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Brucella abortus / genetics*
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Brucella abortus / immunology*
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Brucellosis / prevention & control*
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Gene Dosage
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Genes, Reporter
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Genetic Complementation Test
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Leucine / biosynthesis*
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Leucine / genetics*
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Mice
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Plasmids
Substances
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Brucella Vaccine
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Green Fluorescent Proteins
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Leucine