Abstract
We created three lines of transgenic mice with an integrated rat genomic apolipoprotein E gene fused with the mouse metallothionein I promoter. These lines transcribed rat apoE mRNA in the liver and/or in the kidney and expressed significant amounts of rat apoE in plasma. Enhancement of the plasma level by treatment with Zn ion or Bi ion was observed.
MeSH terms
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Animals
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Apolipoproteins E / blood
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Apolipoproteins E / genetics*
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Base Sequence
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Bismuth / metabolism
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Blotting, Northern
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Blotting, Southern
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Gene Expression Regulation / physiology
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Immunoblotting
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Kidney / metabolism*
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Liver / metabolism*
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Metallothionein / genetics*
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Mice
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Mice, Transgenic
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics*
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Rats
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Recombinant Proteins / biosynthesis
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Zinc / metabolism
Substances
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Apolipoproteins E
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Recombinant Proteins
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Metallothionein
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Zinc
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Bismuth