Testing of experimental compounds in a relapse model of tuberculosis using granulocyte-macrophage colony-stimulating factor gene-disrupted mice

Antimicrob Agents Chemother. 2009 Jan;53(1):306-8. doi: 10.1128/AAC.01346-07. Epub 2008 Oct 13.

Abstract

This study describes an in vivo model for evaluating the sterilizing activity of compounds against persisting Mycobacterium tuberculosis. The initial treatment with isoniazid and rifampin in granulocyte-macrophage colony-stimulating factor gene-disrupted mice reduced the number of bacteria more than 99% within 3 weeks. A subsequent treatment with individual drugs was performed to assess their activity on the 1% of remaining bacilli and disease relapse.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antitubercular Agents / therapeutic use*
  • Bacillus / drug effects
  • Disease Models, Animal
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • Lung / drug effects
  • Lung / microbiology
  • Mice
  • Mice, Mutant Strains
  • Mycobacterium tuberculosis / drug effects*
  • Spleen / drug effects
  • Spleen / microbiology
  • Tuberculosis / drug therapy*
  • Tuberculosis / genetics*

Substances

  • Antitubercular Agents
  • Granulocyte-Macrophage Colony-Stimulating Factor