Detection of Anaplasma marginale rickettsemia prior to onset of clinical signs by using an antigen capture enzyme-linked immunosorbent assay

J Clin Microbiol. 1991 Jul;29(7):1542-4. doi: 10.1128/jcm.29.7.1542-1544.1991.

Abstract

An antigen capture enzyme-linked immunosorbent assay was developed by using monoclonal antibodies to conserved epitopes on the Anaplasma marginale MSP1a surface protein. The assay sensitivity was 1.1 (+/- 0.5)% parasitized erythrocytes, and all infected cattle were detected prior to development of 2.0%-parasitized erythrocytes. Positive tests preceded the onset of anemia by a mean of 2 days. The assay was specific for anaplasmosis, as demonstrated by nonreactivity with another common hemoparasitic pathogens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anaplasmosis / diagnosis*
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Cattle
  • Cattle Diseases / diagnosis*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Evaluation Studies as Topic
  • Sensitivity and Specificity
  • Sepsis / diagnosis
  • Sepsis / veterinary

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial