Objective: To evaluate the antitumor activities against hepatocellular carcinoma induced by bone marrow mesenchymal stem cells(MSCs) from BALB/c mice pulsed with tumor-derived exosomes.
Methods: Exosomes derived from malignant ascites of hepatocellular carcinoma were isolated and purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation from BALB/c mice. Bone marrow MSCs from BALB/c mice were obtained using adherent culture method combining with magnetic cell sorting (MACS). IFN-gamma stimulated MSCs were pulsed with tumor-derived exosomes, Then the stimulated MSCs cocultured with H22 hepatocellular carcinoma for 72 hours, the proliferation of H22 hepatocellular carcinoma cells were evaluated.
Results: The proliferation of H22 hepatocellular carcinoma cells cocultured with MSCs plused with tumor-derived exosomes, were significantly inhibited (P < 0.05).
Conclusion: When bone marrow MSCs were pulsed with tumor-derived exosomes, MSCs showed enhanced antitumor activities against hepatocellular carcinoma. Therefore, MSCs combined with TEX may offer an effective method to induce antitumor activities.