Protective effects of glycyrrhizin in a gut hypoxia (ischemia)-reoxygenation (reperfusion) model

Intensive Care Med. 2009 Apr;35(4):687-97. doi: 10.1007/s00134-008-1334-y. Epub 2008 Oct 25.

Abstract

Purpose: This study investigated the effects of glycyrrhizin, a potent antioxidant, on tissue injury caused by ischemia/reperfusion (I/R) of the gut.

Methods: I/R injury of the intestine was caused by clamping both the superior mesenteric artery and the celiac trunk for 45 min followed by release of the clamp allowing reperfusion for 1 or 6 h.

Results: Administration of glycyrrhizin, significantly reduced the (a) fall of mean arterial blood pressure, (b) mortality rate, (c) myeloperoxidase (MPO) activity, (d) production of pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta)], (e) histological evidence of gut injury, (f) immunoreactivity of nitrotyrosine, (g) poly ADP-ribose (PAR) formation, (h) the expression of ICAM-1 and P-selectin, (i) activation of nuclear factor-kappaB (NF-kappaB) and (j) signal transducer and activator transcription-3 (STAT-3) induced by splanchnic artery occlusion-reperfusion shock.

Conclusions: This study demonstrates that glycyrrhizin exerts multiple protective effects in splanchnic artery occlusion-reperfusion shock.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use*
  • Electroencephalography
  • Gastrointestinal Tract / drug effects*
  • Gastrointestinal Tract / injuries*
  • Gastrointestinal Tract / metabolism
  • Glycyrrhizic Acid / pharmacology*
  • Glycyrrhizic Acid / therapeutic use*
  • Hypertension / drug therapy
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interleukin-1beta / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Neutrophils / metabolism
  • P-Selectin / metabolism
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • Interleukin-1beta
  • P-Selectin
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1
  • Malondialdehyde
  • Glycyrrhizic Acid
  • Peroxidase