Sequestering from host and characterization of sequence of a ribosomal RNA operon (rrn) from "Candidatus Liberibacter asiaticus"

Xiaoling Deng; Jianchi Chen; Huaping Li

doi:10.1016/j.mcp.2008.09.002

Sequestering from host and characterization of sequence of a ribosomal RNA operon (rrn) from "Candidatus Liberibacter asiaticus"

Mol Cell Probes. 2008 Oct-Dec;22(5-6):338-40. doi: 10.1016/j.mcp.2008.09.002. Epub 2008 Oct 7.

Authors

Xiaoling Deng¹, Jianchi Chen, Huaping Li

Affiliation

¹ Laboratory of Citrus Huanglongbing Research, Department of Plant Pathology, South China Agricultural University, Guangzhou, PR China.

PMID: 18955129
DOI: 10.1016/j.mcp.2008.09.002

Abstract

A 5005bp rrn DNA sequence of "Candidatus Liberibacter asiaticus" was obtained by PCR using primers conserved to Rhizobiaceae of alpha-proteobacteria. The rrn locus consisted of a 16S rRNA gene (rrs), an intergenic transcribed spacer (ITS) containing two genes of tRNA(Ile) and tRNA(Ala), a 23S rRNA gene (rrl), an ITS without tRNA gene and a 5S rRNA gene (rrf). Like rrs, rrl and rrf were also related (89-90%) to those of the members of Rhizobiaceae. Interestingly, the genes of tRNA(Ile) and tRNA(Ala) were more related to those of non-Rhizobiaceae alpha-proteobacteria. The non-tRNA gene regions of the 16S-23S ITS and 23S-5S rRNA ITS did not share significant similarity to any known non-Liberibacter DNA sequences, and could be used for specific and efficient detection of "Ca. L. asiaticus".

MeSH terms

Base Sequence
Genes, rRNA*
Molecular Sequence Data
Operon*
RNA, Bacterial* / analysis
RNA, Bacterial* / genetics
RNA, Ribosomal* / analysis
RNA, Ribosomal* / genetics
Rhizobiaceae / genetics*

Substances

RNA, Bacterial
RNA, Ribosomal