Abstract
Maturation of miRNAs by dicer is required in vertebrates for normal neural development. Here we report that dicer inactivation in Xenopus affects cell cycle progression, survival and timing of the generation of retinal cells, resulting in small retinas with lamination defects. In particular, dicer inactivation delays the exit from the cell cycle and the translation of key genes of late neurogenesis, highlighting a crucial role of miRNAs in retinal development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Base Sequence
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Cell Cycle
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Cell Survival
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Gene Silencing
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MicroRNAs / genetics
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MicroRNAs / metabolism
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Oligodeoxyribonucleotides, Antisense / genetics
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Retina / abnormalities
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Retina / cytology
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Retina / growth & development*
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Retina / metabolism
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Ribonuclease III / antagonists & inhibitors*
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Ribonuclease III / genetics
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Stem Cells / cytology
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Stem Cells / metabolism
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Xenopus laevis / genetics*
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Xenopus laevis / growth & development*
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Xenopus laevis / metabolism
Substances
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MicroRNAs
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Oligodeoxyribonucleotides, Antisense
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Ribonuclease III