Evolutionary conservation of the chlorophyll a/b-binding proteins: cDNAs encoding type I, II and III LHC I polypeptides from the gymnosperm Scots pine

Mol Gen Genet. 1991 Sep;229(1):67-76. doi: 10.1007/BF00264214.

Abstract

cDNAs encoding three different LHC I polypeptides (Type I, Type II and Type III) from the gymnosperm Scots pine (Pinus sylvestris L.) were isolated and sequences. Comparisons of the deduced amino acid sequences with the corresponding tomato sequences showed that all three proteins were highly conserved although less so than the LHC II proteins. The similarities between mature Scots pine and tomato Types I, II and III LHC I proteins were 80%, 87% and 85%, respectively. Two of the five His residues that are found in AXXXH sequences, which have been identified as putative chlorophyll ligands in the Type I and Type II proteins, were not conserved. The same two regions of high homology between the different LHC proteins, which have been identified in tomato, were also found in the Scots pine proteins. Within the conserved regions, the Type I and Type II proteins had the highest similarity; however, the Type II and Type III proteins also showed a similarity in the central region. The results suggest that all flowering plants (gymnosperms and angiosperms) probably have the same set of LHC polypeptides. A new nomenclature for the genes encoding LHC polypeptides (formerly cab genes) is proposed. The names lha and lhb are suggested for genes encoding LHC I and LHC II proteins, respectively, analogous to the nomenclature for the genes encoding other photosynthetic proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Evolution
  • Chlorophyll / metabolism
  • Chlorophyll A
  • Light-Harvesting Protein Complexes
  • Molecular Sequence Data
  • Photosynthetic Reaction Center Complex Proteins / genetics*
  • Photosynthetic Reaction Center Complex Proteins / metabolism
  • Plant Proteins / genetics*
  • Plants / genetics*
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid

Substances

  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • Plant Proteins
  • Chlorophyll
  • chlorophyll b
  • Chlorophyll A

Associated data

  • GENBANK/S55262
  • GENBANK/S55264
  • GENBANK/S55272
  • GENBANK/S55527
  • GENBANK/S55528
  • GENBANK/S55529
  • GENBANK/S55530
  • GENBANK/S55531
  • GENBANK/S55532
  • GENBANK/S63909
  • GENBANK/X58514
  • GENBANK/X58515
  • GENBANK/X58516
  • GENBANK/X58517