Preparation and characterization of perhydroxyl-cucurbit[6]uril bonded silica stationary phase for hydrophilic-interaction chromatography

Si-Min Liu; Li Xu; Cheng-Tai Wu; Yu-Qi Feng

doi:10.1016/j.talanta.2004.04.012

Preparation and characterization of perhydroxyl-cucurbit[6]uril bonded silica stationary phase for hydrophilic-interaction chromatography

Talanta. 2004 Nov 15;64(4):929-34. doi: 10.1016/j.talanta.2004.04.012.

Authors

Si-Min Liu¹, Li Xu, Cheng-Tai Wu, Yu-Qi Feng

Affiliation

¹ Department of Chemistry, Wuhan University, Wuhan 430072, PR China.

PMID: 18969692
DOI: 10.1016/j.talanta.2004.04.012

Abstract

In this paper, perhydroxyl-cucurbit[6]uril ((HO)(12)CB[6]) was, for the first time, grafted to silica gel as a hydrophilic-interaction chromatographic stationary phase. Several alkaloids were used as probes to investigate the retention mechanism on the new stationary phase. The effect of mobile phase variables such as acetonitrile content, ionic strength and pH on their chromatographic behavior was investigated. The results indicate that the stationary phase behaves as a hydrophilic-interaction chromatographic packing. Finally, several alkaloids were separated on the stationary phase.