Determination of clenbuterol by capillary electrophoresis immunoassay with chemiluminescence detection

Xinghu Ji; Zhike He; Xinping Ai; Hanxi Yang; Chuanlai Xu

doi:10.1016/j.talanta.2006.02.053

Determination of clenbuterol by capillary electrophoresis immunoassay with chemiluminescence detection

Talanta. 2006 Sep 15;70(2):353-7. doi: 10.1016/j.talanta.2006.02.053. Epub 2006 Apr 11.

Authors

Xinghu Ji¹, Zhike He, Xinping Ai, Hanxi Yang, Chuanlai Xu

Affiliation

¹ College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.

PMID: 18970774
DOI: 10.1016/j.talanta.2006.02.053

Abstract

A competitive immunoassay for clenbuterol (CLB) based on capillary electrophoresis with chemiluminescence (CL) detection was established. The method was based on the competitive reaction of horseradish peroxidase (HRP)-labeled CLB (CLB-HRP) and free CLB with anti-CLB antiserum. The factors affecting the electrophoresis and CL detection were systematically investigated with HRP as a model sample. Under the optimal conditions, the tracer CLB-HRP and the immunoassay complex were separated, and the linear range and the detection limit (S/N=3) for CLB were 5.0-40nmoll(-1) and 1.2nmoll(-1), respectively. The proposed method has been applied satisfactorily in the analysis of urine sample.