Effects of time of collection and sample processing on von Willebrand factor concentration in retired racing greyhounds

J Vet Intern Med. 2008 Nov-Dec;22(6):1447-8. doi: 10.1111/j.1939-1676.2008.0198.x. Epub 2008 Oct 3.

Abstract

Background: Concentrations of von Willebrand factor (vWF) are less than 30% in approximately 10% of Greyhounds.

Hypothesis: That sample collection, processing, and storage can affect the concentration of vWF in plasma of Greyhounds.

Animals: Nineteen healthy former racing Greyhounds.

Methods: Prospective study: Blood samples were obtained from the jugular vein of dogs at 4 times during the day. Samples were divided and processed in each of 3 ways. The 1st tube was centrifuged immediately, the 2nd tube was kept in a household refrigerator, and the 3rd tube was kept at room temperature for 3 hours before centrifugation and processing.

Results: There were no significant differences in the vWF concentration between different sample storage and processing (P = 5.31). There was no statistically significant diurnal variation in vWF concentration in the samples evaluated (P = .37).

Conclusions and clinical importance: Time of sample collection and short-term storage temperature do not influence the vWF concentration in retired racing Greyhounds.

MeSH terms

  • Animals
  • Circadian Rhythm
  • Dogs / blood*
  • Specimen Handling / veterinary*
  • Temperature
  • von Willebrand Factor / metabolism*

Substances

  • von Willebrand Factor