[DNA polymorphism detection of Cannabis using amplified fragment length polymorphism]

Fa Yi Xue Za Zhi. 2008 Oct;24(5):330-2.
[Article in Chinese]

Abstract

Objective: To screen the AFLP primers with good diversity to distinguish various species of Cannabis.

Methods: The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.

Results: A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.

Conclusion: AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis / methods*
  • Cannabis / classification
  • Cannabis / genetics*
  • DNA, Plant / analysis
  • DNA, Plant / genetics*
  • Forensic Genetics
  • Genetic Variation*
  • Polymorphism, Genetic*

Substances

  • DNA, Plant